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The resolved strain was transformed again with the pJV53H to subsequently generate double mutants

This medication was approved by the FDA in January 2020 for the treatment of active TED based on the results of two randomized, double-masked, placebo-controlled, parallel-group, multicenter trials

Refractive Index (RI) and ultraviolet spectroscopy (UV) monitoring in Solid Phase Peptide Synthesis (SPPS): Used during coupling and deprotection, RI detects deviations in reaction completeness or reagent flow, while UV confirms Fmoc removal via characteristic absorbance

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Simple technique