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At 2 months of age, male and female tamoxifen-inducible cellspecific Glp-1r cell+/+ (WT) and Glp-1r cell/ (KO) littermates were placed on a HFD consisting of ground chow (5012 LabDiets) supplemented with 3.4% butter fat, 8.5% tallow, 13.1% soybean oil, 3.5% mineral mix, and 1% vitamin mix (Dyets) for 6 weeks

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25 g protein were analyzed and FMO3 concentration was normalized to the alpha-tubulin quantity

Physioco-chemical analysis of biochar showed 5.4 cmol kg 1 cation exchange capacity, 6.9 ds/m electrical conductivity, pH of 9.6, 0.50 g cm 3 bulk density, and organic carbon 3.64% (Lalay et al