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Advanced analytical techniques are enabling researchers to track the compound's effects on global metabolite profiles, revealing complex interactions between NAD+ metabolism, methylation cycles, and other metabolic pathways that were previously unappreciated

The BRET signal resulting from ligand stimulation was calculated by subtracting the BRET ratio for the vehicle-treated cells from the ligand-stimulated cells of the same aliquot

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Simpson ER, Misso M, Hewitt KN, et al