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Cell transfection for gene overexpression and silencing To achieve overexpression, full-length PCBP1, PCBP2, and GPX4 were inserted into the pcDNA3.1 vector
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In addition, there is no evidence that meningococcus can utilize glucose-6-phosphate, which is an available carbon source in the intracellular environment, as other pathogenic bacteria do (Maloney et al., 1990)