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Primers were obtained from Origene (Rockville, MD, USA), except ACTB, which was adopted from Racz et al

Trends Endocrinol Metab 25(1):4252 Nwia SM, Li XC, Leite APdO, Hassan R, Zhuo JL (2022) The Na+/H+ exchanger 3 in the intestines and the proximal tubule of the kidney: localization, physiological function, and key roles in angiotensin II-induced hypertension

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Inadequate protein during rapid fat loss leads to lean mass loss alongside fat loss, reducing the quality of the final body composition result

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