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The culture supernatants were then discarded and replaced with complete medium containing 10% CCK-8 solution (Beyotime Biotechnology, Shanghai, China), according to the manufacturer's requirements, and the cells were incubated in a 37 C incubator in the dark for 1.5 h

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The metabolite ratios were generated from all unique combinations of the metabolites except 3-carboxy-4-methyl-5-propyl-2-furanpropanoic acid (CMPF), 4-ethylphenylsulfate (4-EPS) and dodecanedioic acid where 90% of the measurements were below the LLOQ

Summary: Retatrutide is not currently available in the UK or globally, as it remains an investigational medicine without marketing authorisation, accessible only through authorised clinical trials as of mid-2025

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