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Green CL, Lamming DW, Fontana L

The evaluation of the frequency of entries into the novel arm, the total entries into all arms, and the time spent in the novel arm serves as an indicator of memory function ( 2.7 Novel-Object Recognition Test The test, formulated by Ennaceur and Delacour, evaluates the duration animals require to exhibit a preference for exploring unfamiliar objects over familiar ones ( 2.8 Elevated plus maze test The EPM test was implemented to investigate anxiety-like behavior in rodents, following the protocol delineated by Handley and Mithani (Naz et al., 2022)

S10) reflect that the models barrier integrity was not changed during the nanocarrier permeability experiment confirming the robustness of the model and the accuracy of the assay