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Lancet 402(10403):693704 Aroda VR, Blonde L, Pratley RE (2022) A new era for oral peptides: SNAC and the development of oral semaglutide for the treatment of type 2 diabetes

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To induce expression of proteins under a doxycycline-inducible promoter (pTRE3G), cells were incubated with medium supplemented with 100200 ng ml 1 doxycycline overnight