High-performance Liquid Chromatography Analysis A LaChrom ELITE system (Hitachi High-Tech Corporation, Tokyo, Japan) was used for the high-performance liquid chromatography (HPLC) analysis, with a L2100 pump, L2200 autosampler, L2300 column oven, and L2455 detector
Total Glutathione Levels and GSH:GSSG Ratio Total glutathione and GSSG were determined using a GSH and GSSG Assay Kit (Beyotime Institute of Biotechnology, Shanghai, China) according to the manufacturers protocol
A dulled needle causes more tissue damage, more pain, and creates a larger opening in the rubber stopper that accelerates coring
Acknowledgements This work was supported by a Grant from the University of Maryland Statewide Health Network and other Tobacco-Related Diseases Research Grant through the Maryland Cigarette Restitution Fund Program
Supplementation with NAC, cysteine, and glycine has been shown to increase GSH levels.*