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Hence, fragment library design or selection plays a foundational role in FBDD by ensuring that screened compounds are diverse, synthetically tractable, and optimised for downstream progression 16

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Likewise, pro-apoptotic genes such as the caspases (CASP9, CASP3 and CASP2) and apoptotic protease activating factor (APAF) are induced during the same period [14]

2.3 Sample collection During the culture period, three bullfrogs were dissected from each group every 5 days to observe their gastrointestinal condition