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Lipid raft recruitment assay INS-1 832/3 SNAP/FLAG-hGLP-1R WT or V229A cells were seeded onto 6-cm dishes and stimulated with 100 nM exendin-4 or vehicle for 2 minutes at 37C, placed on ice, and washed with ice cold PBS before being osmotically lysed with 20 mM Tris-HCl (pH 7.0) supplemented with protease and phosphatase inhibitor cocktails

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So I built it