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Following washing twice with PBS, 10 l Annexin V-fluorescein isothiocyanate and 5 l propidium iodide (BD Biosciences, Franklin Lakes, NJ, USA) were added and incubated for 15 min in the dark at room temperature

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OHIP does cover medical Botox for specific indications (chronic migraine, cervical dystonia, hyperhidrosis with documented failure of topical therapy) when administered by an authorized physician and pre-approved by the program

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For the shoulder, where multiple structures may be involved in a single injury pattern, this enhanced sensitivity helps coordinate healing across different tissue types