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Handling & Preparation Reconstitute using bacteriostatic water per laboratory protocol Use only trained professionals Follow institutional research and safety standards Storage Instructions To preserve potency and stability: Store lyophilized vials in a cool, dry environment Refrigerate reconstituted solutions at 28C Protect from heat, light, and moisture Avoid repeated freezethaw cycles Possible Observations in Research Settings Reported observations may include: Mild redness at administration site Temporary dizziness or headache Transient nausea All findings should be documented and evaluated within controlled research environments

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We calculated Pearsons correlation coefficient to assess correlations between significant GPs (i.e., GPs that showed significant fold changes at the end of 4th week during 4-week intermittent fasting and 1 week after 4-week intermittent fasting) and the components of metabolic syndrome, lipid panel, hepatic panel, and adiposity, oxidative stress, and inflammation biomarkers