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Inside mitochondria, an NAD+-dependent sirtuin named SIRT3 activates enzymes that pump out NADPH specifically to feed glutathione recycling

For imaging, various stages of the transgenic animals were mounted on 5 % agar pads with polystyrene nanoparticles (Polysciences, 2.5 % by volume) 60 and imaged at an AxioImager M.2 fluorescence microscope (Zeiss, Jena, Germany)

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Metabolites can be detected in urine

The PCA of the nine adjusted metabolite concentration values (glutathione, glutamate, and glutamine in each of the three voxels, ACC, Insula, and Visual) resulted in three components with an eigenvalue greater than 1