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Following acquisition of functional and perfusion-related measurements as previously described, all recording instruments were removed, and the procedure was concluded by division of the heart from its thoracic and vascular attachments

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Following the collection of blood, and while the animals were still anesthetized, euthanasia was performed via decapitation and their brains were meticulously removed from their skulls and examined closely

doi: 10.1007/s40520-016-0548-z 226 JiaXTYeTYuanLZhangGJLiuZQDiZLet al