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The PCR program was set as: 98 C for 3 min, 98 C for 10 s, 5560 C (temperature and time were set according to primers), 72 C for 30 s, and a total of 35 cycles

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Synaptic biomarkers, or proteins found in cerebral spinal fluid, have been found to be linked to early indicators of Alzheimer's disease progression in a study that observed over 700 people: Biomarkers linked to excitatory neuron communication, specifically Neuronal Pentraxin (NPTX2), were observed at low levels to determine correlation

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Health care providers and patients must be well-informed of these risks, and patients with a personal or family history of medullary thyroid carcinoma or patients with Multiple Endocrine Neoplasia syndrome type 2 should not use tirzepatide