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doi: 10.1186/s11658-022-00355-3 286 ZendedelA.HabibP.DangJ.LammerdingL.HoffmannS.BeyerC.et al

This can be seen with pre-operative X-rays

Several mechanisms may explain reduced effectiveness over time

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Problem analysis Problem description: The positive and negative control results are unstablePossible causes and corresponding countermeasures: 1.Inaccurate aspiration or addition of liquidCheck the pipette and tips 2.Equilibration time is too shortEnsure adequate balancing time 3.Incomplete washingEnsure the washing time, washing times and liquid volume per well Problem description: The color is very weak or colorlessPossible causes and corresponding countermeasures: 1.Incubation time is too shortEnsure adequate incubation time 2.Incorrect test temperatureUse the recommended test temperature 3.Insufficient reagent volume or missed addition/Incorrect dilutionCheck the aspiration and addition process to ensure that all reagents are added in order and in sufficient quantities