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Peptides were injected onto a 75 m 2 cm trap column (Thermo Fisher) before re-focusing on an Aurora Elite 75 m 15 cm C18 column (IonOpticks) using a Vanquish Neo UHPLC (Thermo Fisher) connected to an Orbitrap Astral mass spectrometer (Thermo Fisher)

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BSO treatment was not included, because this already induced a significant oxidative stress response (data no shown)

Hellewell SB, Bruce A, Feinstein G, Orringer J, Williams W, Bowen WD