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Digested tissue was centrifuged at 120 g and the resulting pellet was re-suspended in DMEM containing 5.6 mM glucose (Cat

The prevalence of diabetes-associated microvascular complications across both cohorts was low (34%)

Geographic factors also influence the decision

Immediate medical attention is required if patients experience severe abdominal pain, particularly if localized, persistent, or accompanied by fever, which may indicate complications such as bowel obstruction, fecal impaction, or rarely, intestinal ischemia