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However, in Drosophila , increased ETC complex I activity extended lifespan apparently through an oxidation of both the mitochondrial and cytoplasmic [NAD + ]/[NADH] to increase cytoplasmic NAD + levels to activate nucleocytoplasmic sirtuins to override the potential pro-aging effects of oxidation of the mitochondrial [NAD + ]/[NADH] on longevity (Hur et al., 2014)

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With open cholecystectomy, a large cut is made in the abdomen

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Very high levels, however, can be harmful, so balance is key