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Subsequently, the peptides were separated on a Pepmap100 RSLC C18 column (2.0 m, 75 m 150 mm, Thermo Fisher Scientific) using a multistep gradient of 2% to 40% acetonitrile with 0.1% formic acid over a period of 150 min

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Its a naturally occurring hormone in your body that plays a crucial role in regulating appetite and blood sugar