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The difference between 95% and 99% purity might seem small, but that 4% represents impurities that can include unreacted precursors, truncated sequences, degradation products, and synthesis byproducts

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Xaa is a neutral aliphatic amino acid residue, in particular Ala, bAla, Leu, Ile, Gly, Val, Nle or the cation of the salt is an alkali metal or an alkaline earth metal, for instance Na + , K + , Li + , Cs + , Ca 2+ , or another metal, such as Zn 2+ , or a primary, secondary or tertiary amine or organic compound, such as NH 4 + , triethanolamine + , cyclohexylamine + , 2-AMP + (2-amino-1-propanol) or TRIS + (Tris-(hydroxymethyl)-aminomethan), as long as these cations are physiologically acceptable

Purity testing typically uses High Performance Liquid Chromatography to identify the peptide and assess the concentration of the target compound versus impurities