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The resulting cells were fixed in 4% paraformaldehyde and incubated with a blocking buffer for 1 h, and the cells were permeabilized with 0.2% Triton and 0.1% Tween 20 in a blocking buffer (3% bovine serum albumin in PBS) for 2 h

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Biomolecules 12:1120

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Consideration: While it may aid in weight loss for some people, it should be considered a supplement to, not a replacement for, a thorough diet and exercise regimen developed with professional nutritional counseling