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The HT+GSH treatment enhanced P n by a factor of 2.3 compared to HT

It is produced and secreted by specific cells in the intestine and certain neurons in the brainstem when food is consumed

The protein was then incubated with a buffer consisting of 20 mM HEPES, pH 7.4, 100 mM NaCl, 10% (v/v) glycerol, 5 mM MgCl 2 , 1 mM MnCl 2 , 25 M TCEP, 50 M tirzepatide, 20 g/mL Nb35, 0.03% (w/v) LMNG, 0.01% (w/v) GDN, 0.008% (w/v) CHS and 30 g/mL His-tagged TEV protease on the column overnight at 4 C