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Nuclear factor-B activation contributes to vascular endothelial dysfunction via oxidative stress in overweight/obese middle-aged and older humans

Avoid placing patches on areas with scars, cuts, or irritated skin

Briefly, cells were lysed using the RIP lysis buffer provided in the kit, and cell lysates were incubated with magnetic beads pre-coated with either anti-SYNCRIP antibody (R5653, Sigma-Aldrich) or purified mouse IgG (5415, Cell Signaling Technology) as a negative control