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In addition to these structural artifacts, the cell-type homogeneity in BEC monolayers does not accurately recreate the BBB and BTB microenvironment and molecular characteristics

The inclusion of benzyl alcohol serves as a bacteriostatic preservative, which inhibits the growth of potentially contaminating bacteria

Appropriate secondary antibodies conjugated to Alexa-Fluor 488, 555 or 633 (Supplementary Table 1) were incubated at 1:300 for 2 h before mounting on microscope slides using Prolong Gold

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Do not stop the drug abruptly yourself