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A few have real human evidence, usually outside the brain, and most have little brain evidence at all

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2.6 Measures of reactive oxygen species (ROS) by the 2,7-dichlorofluorescein diacetate (DCFH-DA) assay 2,7-dichlorofluorescein diacetate (DCFH-DA) was used as probe for the estimation of ROS content in tissue homogenate