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Thermodynamic Parameters for Salt-Induced Reversible Protein Precipitation from Automated Microscale Experiments

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Understanding reconstitution, calculation methods, and administration techniques supports reliable protocol implementation

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Proteins were separated by SDS-PAGE at 100 V for 90 min in Tris/glycine/SDS running buffer, then transferred onto polyvinylidene difluoride (PVDF) membranes (#1,620,177, BIO-RAD, Warsaw, Poland) using a wet transfer system at 100 V, 250 mA for 1 h at 4 C in Tris/glycine buffer containing methanol, as previously described by Alicka M