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Next, the cell medium was replaced, and 100 L of different doses of AP probe-net (final concentration: 100, 75, 50, 25, 12.5, 6.25, 3.12 and 1.56 M) were added and incubated for another 24 h and 48 h, respectively

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The crossing of these cellular barriers is essential for the development of the IMD, and although it has been intensively studied, it remains not fully understood

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