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ORIGIN reported a 1.6 kg gain over 6 years with insulin glargine [46]

After three washes with PBS, sections were incubated with 4,6-diamidino-2-phenylindol (DAPI, 1:10,000 in PBS) at room temperature for 10 min

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Buse JB, Klonoff DC, Nielsen LL et al (2007) Metabolic effects of two years of exenatide treatment on diabetes, obesity, and hepatic biomarkers in patients with type 2 diabetes: an interim analysis of data from the open-label, uncontrolled extension of three double-blind, placebo-controlled trials