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4.11 Total proteomics and phosphoproteomics analysis by liquid chromatography combined with tandem mass spectrometry Be(2)-C cells were cultured in triplicates in 10 cm dishes and treated the following day either with MitoQ IC50, DPI IC50, Vin IC50, (M+D)/2, (M+D+V)/3, or vehicle for 24 h and washed once with PBS

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This is different than some other hyaluronic acid fillers, such as Restylane, which does not attract water

The proteins present in the plasma samples were precipitated using three volumes of ice-cold acetonitrile

This keeps out air, dust, and other potential contaminants