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First, although we initially planned to include five mice per group, one animal in the tirzepatide-treated group died just before drug administration (day 0), and an additional mouse died on day 10 after treatment initiation

Interestingly, one month after the period of APAP intoxication, these mice showed increased glial burden without associated drivers, namely, bloodbrain barrier disruption, cholesterol accumulation, and elevation of inflammatory molecules in the brain and/or periphery

However, this study had limited duration of drug use and a lack of controls in the regression model for wet AMD risk factors, such as baseline PDR and DME status, smoking history, hemoglobin A1c, and history of dry AMD

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig IL8