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Furthermore, we determined the affinity between PCBP1 and ACE via surface plasmon resonance (SPR), and the results revealed that ACE bound directly to PCBP1 with a KD value of 0.8464 M (Fig

Considering the bio-sourced materials are quite low-cost and biocompatible, we believe the well-designed MN-array patch can serve as an economic, convenience, safe, and highly effective candidate for long-term T2D treatments

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Competing Interests None

Protein expression and purification SLC33A1 and SLC33A1 MAP Genes encoding human SLC33A1 and SLC33A1 MAP were subcloned into a pBacMam expression vector with C-terminal eGFP tag fused through a short linker containing a PreScission protease site 58