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The involvement of miR-25 in the regulation of apoptotic marker genes via downregulation followed by recovery of response upon reconstitution, as well as the EGCG-mediated induction of apoptosis and interference with cell cycle progression (G2/M block), provide evidence that miR-25 may be a regulator of the apoptotic effects created by EGCG in these cells

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Protein expression and purification Expression and purification of TRF1 TRFH domains was carried out as described previously with some modifications 15,31