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The vector was linearized with PacI and was cotransfected with pACAd-9.2-100 into 293AD cells (Cell Biolabs, AD-100) according to the manufacturers protocol (Cell Biolabs, VPK-254)

[DOI] [PMC free article] [PubMed] [Google Scholar] Berndt C, Christ L, Rouhier N, Mhlenhoff U

I was really nervous about the possibility of nausea and other side affects but after speaking in depth with them and starting the shot I am in shock that I not only dont experience nausea but Im actually losing weight

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