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EPN-1::GFP is readily visualized in all four blastomeres at the four-cell stage of embryogenesis, and RNAi efficiently reduces EPN-1::GFP to undetectable levels at the 4-cell stage (Figure 1B, left)

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Cleft lip repair:Technical refinements for the wide cleft

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Afterwards aliquots of the cell suspension were treated with 10 mM DTT for 10 min at 25C and the reductant was washed out by three washing steps with HEPES buffer