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Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association 60 , 3844, doi: 10.1016/j.fct.2013.07.008 (2013)

Murine cells were simultaneously fixed and permeabilized in Foxp3 Fixation/Permeabilization working solution for 60 min at RT while protected from light

As de novo synthesis of ceramide is located in the ER, which is absent in erythrocytes, it is thus assumed that erythrocytic ceramide should directly originate from the hydrolysis of sphingomyelin

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