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1 x 10 6 lung metastatic derived PDAC cells were then nucleoporated using a Nucleofector 2b Device (AAB-1001, Addgene) using the Amaxa Cell Line Nucleofector Kit R (VCA-1001, Lonza) and plated into a 10 cm tissue culture dish

Osmotic Diuretics (e.g., Mannitol): Increase osmotic pressure in the tubules, used in cerebral edema and acute kidney injury