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RT-PCR RNA was extracted from fully matured HBE cells with the AllPrep DNA/RNA Mini kit (80204, QIAGEN AG, Germantown, MD) and treated with DNase I (M0303S, New England BioLabs Inc., Ipswich, MA) before reverse transcription with the iScript cDNA Synthesis Kit (1708891, Bio-Rad)

Updated October 28, 2024

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Between 2019 and 2020, the CDC documented 2,807 hospitalizations and 68 deaths from EVALI, nearly all linked to vitamin E acetate in unregulated THC cartridges the exact contaminant Cyclone's lab panel tests for

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