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In 2020, FDA announced a new policy to prioritize enforcement of the pre-market authorization requirement for any flavored ENDS product (other than tobacco or menthol) and all other ENDS products likely to target minors

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The genomic DNA was isolated from the spermatozoa using the QIAamp DNA Mini Kit, followed by mitochondrial DNA amplification via the REPLI-g Mitochondrial DNA Kit supplied by QIAGEN (Hilden, Germany), adhering to the kits instruction manual