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Pace G, Loureno J, Ribeiro CA, Rodrigues C, Pascoal C, Cssio F (2024) Spatial accumulation of flood-driven riverside litter in two Northern Atlantic Rivers

Paraffin blocks were sectioned onto SuperFrost Plus slides, dried overnight, and baked for 1 h at 60 C before immediately proceeding with the RNAScope Multiplex Fluorescent v2 assay according to the manufacturers instructions with the following modifications: Target retrieval was performed for 15 min in a boiling beaker, Protease III was used for 30 min pre-treatment at 40 C and hybridized slides were left overnight in 5 SSC before proceeding with the amplification and labeling steps

Running Time Approximately 1hr 14min

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These situations are difficult, and we respect the real-world challenges posed by wildfires in the Pacific Northwest, Hawaii, and other areas