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First, cells were pelleted, the supernatant was removed, and the cell pellet again resuspended in 500 L of HBSS/BSA/EDTA buffer (0.04% BSA and 2 mM EDTA in HBSS)

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Jacobsen, The Influence of Dietary Tea, Coffee and Cocoa on Protein and Energy Utilization of Soya-bean Meal and Barley in Rats, British Journal of Nutrition, September 1983;50(2):197205