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The gene-specific primers and probes used were: Rps3 as endogenous control (Life Technologies assay number Mm00656272_m1), interleukin 1 beta ( Il1b) (forward 5-AAATACCTGTGGCCTTGGGC-3, reverse 5-TCT TCTTTGGGTATTGCTTGGGA-3), tumor necrosis factor-alfa ( Tnfa) (forward 5-GTAGCCCACGTCGTAGCAAA-3, reverse 5-TTGAGATCCATGCCGTTGGC-3), Il4 (forward 5-TCGGC ATTTTGAACGAGGTC-3, reverse 5-CTGTGGTGTTCTTCG TTGCTG-3), Il5 (forward 5-TTGACCGCCAAAAAGAGAA GTG-3, reverse 5-CTCAGCCTCAGCCTTCCATT-3), Il13 (forward 5-AGCATGGTATGGAGTGTGGAC-3, reverse 5-GC TGGAGACCGTAGTGGGG-3), Il17 (forward 5-GGACTCTC CACCGCAATGAA-3, reverse 5-TTTCCCTCCGCATTGACA CA-3), Il25 (forward 5-TGGAGCTCTGCATCTGTGTC-3, reverse 5-GATTCAAGTCCCTGTCCAACTC-3), and Il33 (forward 5-GGTGAACATGAGTCCCATCA-3, reverse 5- CGTCACCCCTTTGAAGCTC -3)

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To test whether the CD4 + CD45RO + circulating ILC3s are maintained after quitting smoking (i.e., whether they display a memory function), we divided the asthma patients according to their current smoking status (non-smokers, former smokers, and current smokers)

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