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Lonza) were seeded onto collagen-coated 12 mm transwell with 0.4 m pore size and differentiated under ALI culture for 28 days in medium with 1:1 mixture of BEBM (Lonza) and DMEM (Gibco) supplemented with 52 g/ml bovine pituitary extract, 5 g/ml insulin, 10 g/ml transferrin, 0.5 g/ml hydrocortisone, 0.5 ng/ml human epidermal growth factor, 0.5 g/ml epinephrine, 1.5 g/ml bovine serum albumin and 15 ng/ml retinoic acid

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