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Concurrently, above samples were treated with RNase A (1 g/mL) at 37 C for 15 min, and the total RNA was extracted from intact TMV particles using the EasyPure Viral DNA/RNA Kit (China) and reverse transcribed using the PrimeScriptTM RT reagent Kit (Japan) to obtain the cDNA

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Im breaking a sweat but Im not going nuts, he said