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Exclusive and dual use of electronic cigarettes among European youth in 32 countries with different regulatory landscapes

Plasmids pIVEX1.3_eYFP-His_Poly(A) and pIVEX_GAA_Omega_eYFP-His_Poly(A) were generated by amplifying the 3-UTR from pIX4.0_His-eYFP by PCR using primers 22 and 23 and inserting the product into the KpnI and EcoRI sites of pIVEX1.3_eYFP-His and pIVEX_GAA_Omega_eYFP-His

Based on these mechanisms, experimental studies and cohort studies that consider additional confounders are needed