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PCR products were purified either as pooled amplicons or following agarose gel separation using QIAquick columns (Qiagen), cloned into the pJET1.2 vector (Thermo Fisher Scientific), and transformed colonies were pooled for Oxford Nanopore Technologies (ONT) sequencing

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IU football coach Curt Cignetti has agreed to a new eight-year contract worth an average of $11.6 million annually, the athletic department announced today

Previous studies have shown highly nicotine-dependent individuals have significantly more cotinine, the major breakdown product of nicotine, in their blood