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10.1017/S0033291713003206 11

These methods include thracheostomy, in which smoke is pumped through a tube directly into a hole cut in the dog's throat, or through a mask fitted to the dog's face.- source: Animal and Tobacco Smoke and Smoking Experiment on Animals Dogs, which cannot be exposed to cigarette smoke via inhalation chambers as easily as can small rodents, require different methods of cigarette smoke exposure

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PBECs were stored in liquid nitrogen until use at passage 4 and cultured in bronchial epithelium growth medium (AEGM