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A 25 L reaction mixture was prepared, comprising 12.5 L PCR Master Mix (2X) (Thermo Scientific), 0.8 L of 10 mM forward primer, 0.8 L of 10 mM reverse primer, 2 L MT-DNA (20 ng/L), and 8.9 L nuclease-free water
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"Chapter 5, How Party Systems Develop in Mixed Electoral Systems"
Federal Regulations At the federal level, the Tobacco and Vaping Products Act (TVPA) regulates the sale of tobacco and vaping products in Canada
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