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Fresh medium was added 3060 minutes before transfection

The e-cigarette use exposure variable was defined as a four-level gradient variable (never versus ever use with no use in past 30 days [prior use] versus 12 days in past 30 [current infrequent use] versus 3 days in past 30 [current frequent use])

A user could inhale it and produce enough vapor to get their nicotine fix

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"The best way Philip Morris could help people to stop smoking is to stop making cigarettes," British non-profit Cancer Research told Reuters in 2018